Figure 5
From: Expression of human α1-proteinase inhibitor in Aspergillus niger
Evaluation of stability of r-α1-PI from A. niger (solid trace) and from E. coli (dashed trace) by SE-HPLC. The fractions of r-α1-PI were collected by HPLC, kept on ice and re-injected. The gray dashed trace reflects polymerization of r-α1-PI from E. coli as shown 1.5 h later after elution, while no aggregation was observed for r-α1-PI from A. niger for ~12 hours; all analytes were stored at 4°C.