Metabolic transcription analysis of engineered Escherichia coli strains that overproduce L-phenylalanine

Table 2 E. coli strains and plasmids used for the construction of L-phenylalanine overproducing strains in this study.

Strains	Relevant features	Reference number
JM101	supE, thi, Δ(lac-proAB), F'	70
PB11	Derived from JM101, but ΔptsHI-crr; impaired growth on glucose as sole carbon source.	2
PB12	Derived from PB11; PB12 grows faster than PB11 on glucose	2
PB13	Derived from PB11; PB13 grows faster than PB11 on glucose.	2
Plasmids
pCL *tkt*	tktA (comes from replicon pCL1920, resistant to streptomycin or spectinomycin.	6
pJLB *aroG* ^fbr	aroG^fbrunder the control of lacUV5 promoter, lacI^q and tet genes. Replication origin from pACYC184.	15
pTrc *pheA* ^ev2	Evolved feedback insensitive pheA^ev2under the control of lacUV5 promoter. Ev2 superscript means 2^nd version of evolved pheA^fbrgene.	15
Engineered strains
JM101-ev2	JM101 transformed with pJLBaroG^fbr, pCLtkt and pTrcpheA^ev2	15
PB12-ev2	PB12 transformed with pJLBaroG^fbr, pCLtkt and pTrcpheA^ev2	15
PB13-ev2	PB13 transformed with pJLBaroG^fbr, pCLtkt and pTrcpheA^ev2	69

ISSN: 1475-2859