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Table 2 E. coli strains and plasmids used for the construction of L-phenylalanine overproducing strains in this study.

From: Metabolic transcription analysis of engineered Escherichia coli strains that overproduce L-phenylalanine

Strains Relevant features Reference number
JM101 supE, thi, Δ(lac-proAB), F' 70
PB11 Derived from JM101, but ΔptsHI-crr; impaired growth on glucose as sole carbon source. 2
PB12 Derived from PB11; PB12 grows faster than PB11 on glucose 2
PB13 Derived from PB11; PB13 grows faster than PB11 on glucose. 2
Plasmids
pCL tkt tktA (comes from replicon pCL1920, resistant to streptomycin or spectinomycin. 6
pJLB aroG fbr aroGfbrunder the control of lacUV5 promoter, lacIq and tet genes. Replication origin from pACYC184. 15
pTrc pheA ev2 Evolved feedback insensitive pheAev2under the control of lacUV5 promoter. Ev2 superscript means 2nd version of evolved pheAfbrgene. 15
Engineered strains
JM101-ev2 JM101 transformed with pJLBaroGfbr, pCLtkt and pTrcpheAev2 15
PB12-ev2 PB12 transformed with pJLBaroGfbr, pCLtkt and pTrcpheAev2 15
PB13-ev2 PB13 transformed with pJLBaroGfbr, pCLtkt and pTrcpheAev2 69