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Table 3 Nucleotide sequences of the primers sets used for RT-PCR assays. The rest of the primer sets has been already reported [18].

From: Metabolic transcription analysis of engineered Escherichia coli strains that overproduce L-phenylalanine

Gene

Primer

Primer sequence

Aromatic amino acid pathways

aroF

aroFa

5' CAGAATCCGTGGTTGCTCAA 3'

 

aroFb

5' CGGTTGCTCGGAAGACTGAT 3'

aroG

aroGa

5' GTCGGCTTCAAAAATGGCAC 3'

 

aroGb

5' CCCCATTTCGTTACGGACAG 3'

aroH

aroHa

5' CTGCTCGATCCACGATCTCA 3'

 

aroHb

5' AGGTGCGCATTACGATTTCC 3'

aroB

aroBa

5' GTTATCCTCCCTGACGGCG 3'

 

aroBb

5' ACCAGCGTAGTATCGCGACC 3'

aroD

aroDa

5' AGCCGAAGAAATCATTGCCC 3'

 

aroDb

5' ACGTCAGCACATCGCTGGTA 3'

aroE

aroEa

5' AATCCGATAGCCCACAGCAA 3'

 

aroEb

5' TCATTGATGGGTGCCAACAC 3'

ydiB

ydiBa

5' AAAAGCGGGATTGCCATTTA 3'

 

ydiBb

5' CAGTTCCGCGCATTTTGAG 3'

aroL

aroLa

5' CCGGAAGAAGATTTACGGCC 3'

 

aroLb

5' TGCGCAACTTCGCGATATAG 3'

aroK

aroKa

5' GCACTATTGGGCGCCAGTTA 3'

 

aroKb

5' GAAAACCCAGCCCACATCAG 3'

aroM

aroMa

5' CCAGAAGCAGGCGAAGACAC 3'

 

aroMb

5' TCAACCACACCTTGCAGGTC 3'

aroA

aroAa

5' CATATTCCTGATGCGGCGAT 3'

 

aroAb

5' TCGGTCTCTTTAACACGCCAG 3'

aroC

aroCa

5' GGGACATCGCGCTATACCAC 3'

 

aroCb

5' AACAAGCCAATGCTGGTGC 3'

pheA

pheARa

5' ATGACCCGTCTGGAATCACG 3'

 

pheARb

5' GCTTTTTGCATTTCCGCTG 3'

pheA-ev2

pheAa

5' ACTCAGCAGGCTTTGCTCCA 3'

 

pheAb

5' GCCGCAAGATGGGAATAAGAA 3'

tyrA

tyrAa

5' GTCAGATGGGACGCCTGTTC 3'

 

tyrAb

5' GGCAACAATATCAGCCGCTC 3'

aspC

aspCa

5' GGCGATTCGCGCTAACTACT 3'

 

aspCb

5' GCTCTTGTTCCCAAATCGCA 3'

trpE

trpEa

5' CGCAACTGTCAGCGGAAAAT 3'

 

trpEb

5' GGCCTGAATACGGGTGCTTT 3'

trpA

trpAa

5' CGTTCCTTTCGTCACGCTC 3'

 

trpAb

5' CGTTCCTTTCGTCACGCTC 3'

Aromatic amino acid transport

pheP

phePa

5' CGGCTGTATTGCTGGGCTAC 3'

 

phePb

5' AAATGAACCGGATACCGGCT 3'

aroP

aroPa

5' TGGTAATCAACTGGGCGATG 3'

 

aroPb

5' CGGATAAAGCAGAGCAGGGA 3'

tyrP

tyrPa

5' CCGGTGTTGGTTTTAGCGTT 3'

 

tyrPb

5' CGGAACATGCTGGTACACCTC 3'

mtr

mtra

5' TTGATGTGCTGGTACAGGCG 3'

 

mtrb

5' GAGGAACGAACTCGCTACCG 3'

shiA

shiAb

5' ATTATCAAGCTGCCGCGAAA 3'

 

shiAb

5' CAATTCGCACAGTCGTAGCG 3'

ilvE

ilvEa

5' AAGGCATCCGTTGCTACGAC 3'

 

ilvEb

5' CGGGAAGCGATAGATTTTGG 3'

aroP

aroPa

5' TGGTAATCAACTGGGCGATG 3'

 

aroPb

5' CGGATAAAGCAGAGCAGGGA 3'

Regulators

trpR

trpRa

5' CAGAACAGCGTCACCAGGAG 3'

 

trpRb

5' TGGCGTCAGCATCAGGTTTA 3'

tyrR

tyrRa

5' GGCGAGCTGTCAGCTTTTTG 3'

 

tyrRb

5' CGCTTTCCAGCCAACGTAAA 3'

csrA

csrAa

5' TCTGACTCGTCGAGTTGGTGA 3'

 

csrAb

5' GGGCATTTACGCCAATACGT 3'

rpoD

rpoDa

5' GATTCTGCGACCACCGAAAG 3'

 

rpoDb

5' TCGATACCGAAACGCATACG 3'

rpoE

rpoEa

5' GAACTATTGAGTCCCTCCCGG 3'

 

rpoEb

5' CGGACAATCCATGATAGCGG 3'

fnr

fnra

5' CGGAAAAGCGAATTATACGGC 3'

 

fnrb

5' TTCGTTGAGTGTGAACGGGA 3'

Carbohydrate transport

malE

malEa

5' AGAAATTCCCACAGGTTGCG 3'

 

malEb

5' TTCAGCCAACAGGCCAGATT 3'