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Table 1 Accumulation of aromatic intermediates, acetate, L-Phe yields and productivities in L-Phe overproducing strains assessed in resting cell cultures containing 1 g of biomass. The results of the JM101-ev2, PB12-ev2 and PB13-ev2 strains have been previously reported [15, 69].

From: Metabolic transcription analysis of engineered Escherichia coli strains that overproduce L-phenylalanine

Strains qGlc (g/gDCW·h) qAcetate (mg/gDCW·h) DHS (g/L) SHIK (g/L) L-Phe (g/L) Y Phe/Glc qPhe (mg/gDCW·h)
JM101-ev2 0.17 ± 0.01 70 ± 0.41 0.020 ± 0.001 0.029 ± 0.002 0.396 ± 0.025 0.22 ± 0.01 36.34 ± 2.50
PB12-ev2 0.12 ± 0.01 30 ± 0.22 0.054 ± 0.003 0.034 ± 0.001 0.460 ± 0.015 0.33 ± 0.01 40.60 ± 3.17
PB13-ev2 0.14 ± 0.01 40 ± 0.25 0.054 ± 0.004 0.034 ± 0.002 0.300 ± 0.019 0.20 ± 0.02 29.25 ± 2.50
  1. Symbols: qGlc, specific consumption rate of glucose; qAcetate, specific consumption rate of acetate; qPhe, specific production rate of phenylalanine; YPhe/Glc, yield of phenylalanine synthesized from glucose. DHS, 5-dehydroshikimate; SHIK, shikimate; gDCW, grams of dry cell weight.