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Strains
| | |
|
E. coli DH5α
| | |
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E. coli E10
| | |
|
L. lactis NZ9000
|
MG1363 pepn::nisRK
|
[35, 36]
|
|
L. plantarum WCFS1
|
Sequenced wild-type strain single colony isolate of NCIMB8826 form human saliva.
|
[10]
|
|
NZ7100
|
L. plantarum WCFS1 derivative with chromosomal integration of pEMnisRK plasmid.
|
This work
|
|
NZ7606
|
CmR, L. plantarum NZ7100 derivative carrying the pNZ8150 plasmid.
|
This work
|
|
NZ7601
|
CmR, L. plantarum NZ7100 derivative carrying the pMS011 plasmid.
|
This work
|
|
NZ7607
|
CmR, L. plantarum WCFS1 derivative carrying the pNZ7021 plasmid.
|
This work
|
|
NZ7602
|
CmR, L. plantarum WCFS1 derivative carrying the pMS040 plasmid.
|
This work
|
|
Plasmids
| | |
|
pUC18Ery
|
AmpR, EmR
|
[26]
|
|
pACYC184
|
CmR, TetR
|
[37]
|
|
pNZ84
|
CmR, pACYC184 derivative with deletion of Tetracycline resistance gene and BamhI site.
|
[38]
|
|
pNZ9521
|
EmR, nisRK cloned in pIL253, expression of nisRK driven by rep read-through.
|
[27]
|
|
pNZ7130
|
AmpR, EmR pUC18EM derivative carrying 1.0 kb DNA fragments of both L. plantarum WCFS1 lp_0075 and lp_0077 genes.
|
This work
|
|
pNZ7131
|
AmpR, EmR, pNZ7130 derivative with extra cloning sites Nsa1 and NsiI.
|
This work
|
|
ppNZ7132
|
CmR, Em R, pNZ84 derivative carrying 1.0 kb DNA fragments of L. plantarum WCFS1 (lp_0075) and (lp_0077) genes and the EryR resistance marker.
|
This work
|
|
pNZ7133
|
CmR, Em R p7132 derivative carrying the whole genes nisRK from L. lactis.
|
This work
|
|
pNZ8150
|
CmR, pNZ8148 derivative lactococcal cloning and expression vector with nisA promoter upstream of a multiple cloning site and Sca1 restriction site.
|
[39]
|
|
pMS011
|
CmR, pNZ8150 derivative carrying L. plantarum (lp_0761) trxB1 gene, translation fused to the nisA promoter.
|
This work
|
|
pNZ7021
|
CmR, pNZ8148 derivative carrying nisA::pepN.
|
[40]
|
|
pMS040
|
CmR, pNZ7021 derivative carrying L. plantarum lp_0761 trxB1 gene, translational fused to the pPEPN promoter.
|
This work
|
