Table 5 Bacterial strains and plasmids used in this study.

Strains

E. coli DH5α

E. coli E10

L. lactis NZ9000

MG1363 pepn::nisRK

[35, 36]

L. plantarum WCFS1

Sequenced wild-type strain single colony isolate of NCIMB8826 form human saliva.

[10]

NZ7100

L. plantarum WCFS1 derivative with chromosomal integration of pEMnisRK plasmid.

This work

NZ7606

Cm^R, L. plantarum NZ7100 derivative carrying the pNZ8150 plasmid.

This work

NZ7601

Cm^R, L. plantarum NZ7100 derivative carrying the pMS011 plasmid.

This work

NZ7607

Cm^R, L. plantarum WCFS1 derivative carrying the pNZ7021 plasmid.

This work

NZ7602

Cm^R, L. plantarum WCFS1 derivative carrying the pMS040 plasmid.

This work

Plasmids

pUC18Ery

Amp^R, Em^R

[26]

pACYC184

Cm^R, Tet^R

[37]

pNZ84

Cm^R, pACYC184 derivative with deletion of Tetracycline resistance gene and BamhI site.

[38]

pNZ9521

Em^R, nisRK cloned in pIL253, expression of nisRK driven by rep read-through.

[27]

pNZ7130

Amp^R, Em^R pUC18EM derivative carrying 1.0 kb DNA fragments of both L. plantarum WCFS1 lp_0075 and lp_0077 genes.

This work

pNZ7131

Amp^R, Em^R, pNZ7130 derivative with extra cloning sites Nsa1 and NsiI.

This work

ppNZ7132

Cm^R, Em ^R, pNZ84 derivative carrying 1.0 kb DNA fragments of L. plantarum WCFS1 (lp_0075) and (lp_0077) genes and the Ery^R resistance marker.

This work

pNZ7133

Cm^R, Em ^R p7132 derivative carrying the whole genes nisRK from L. lactis.

This work

pNZ8150

Cm^R, pNZ8148 derivative lactococcal cloning and expression vector with nisA promoter upstream of a multiple cloning site and Sca1 restriction site.

[39]

pMS011

Cm^R, pNZ8150 derivative carrying L. plantarum (lp_0761) trxB1 gene, translation fused to the nisA promoter.

This work

pNZ7021

Cm^R, pNZ8148 derivative carrying nisA::pepN.

[40]

pMS040

Cm^R, pNZ7021 derivative carrying L. plantarum lp_0761 trxB1 gene, translational fused to the pP_EPN promoter.

This work