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Table 5 Bacterial strains and plasmids used in this study.

From: Thioredoxin reductase is a key factor in the oxidative stress response of Lactobacillus plantarum WCFS1

Strain and Plasmids Characteristics Reference
Strains   
E. coli DH5α   
E. coli E10   
L. lactis NZ9000 MG1363 pepn::nisRK [35, 36]
L. plantarum WCFS1 Sequenced wild-type strain single colony isolate of NCIMB8826 form human saliva. [10]
NZ7100 L. plantarum WCFS1 derivative with chromosomal integration of pEMnisRK plasmid. This work
NZ7606 CmR, L. plantarum NZ7100 derivative carrying the pNZ8150 plasmid. This work
NZ7601 CmR, L. plantarum NZ7100 derivative carrying the pMS011 plasmid. This work
NZ7607 CmR, L. plantarum WCFS1 derivative carrying the pNZ7021 plasmid. This work
NZ7602 CmR, L. plantarum WCFS1 derivative carrying the pMS040 plasmid. This work
Plasmids   
pUC18Ery AmpR, EmR [26]
pACYC184 CmR, TetR [37]
pNZ84 CmR, pACYC184 derivative with deletion of Tetracycline resistance gene and BamhI site. [38]
pNZ9521 EmR, nisRK cloned in pIL253, expression of nisRK driven by rep read-through. [27]
pNZ7130 AmpR, EmR pUC18EM derivative carrying 1.0 kb DNA fragments of both L. plantarum WCFS1 lp_0075 and lp_0077 genes. This work
pNZ7131 AmpR, EmR, pNZ7130 derivative with extra cloning sites Nsa1 and NsiI. This work
ppNZ7132 CmR, Em R, pNZ84 derivative carrying 1.0 kb DNA fragments of L. plantarum WCFS1 (lp_0075) and (lp_0077) genes and the EryR resistance marker. This work
pNZ7133 CmR, Em R p7132 derivative carrying the whole genes nisRK from L. lactis. This work
pNZ8150 CmR, pNZ8148 derivative lactococcal cloning and expression vector with nisA promoter upstream of a multiple cloning site and Sca1 restriction site. [39]
pMS011 CmR, pNZ8150 derivative carrying L. plantarum (lp_0761) trxB1 gene, translation fused to the nisA promoter. This work
pNZ7021 CmR, pNZ8148 derivative carrying nisA::pepN. [40]
pMS040 CmR, pNZ7021 derivative carrying L. plantarum lp_0761 trxB1 gene, translational fused to the pPEPN promoter. This work