Figure 7
From: Production of Recombinant Peanut Allergen Ara h 2 using Lactococcus lactis
Immune reactivity of rAra h 2. Sandwich ELISA using sera from rabbits immunized with purified native Ara h 2. Concentrations on the primary axis refers to protein concentration of peanut extract, rAra h 2, and nuclease. The L. lactis produced rAra h 2 was run in two-fold dilutions as was the negative protein control (an L. lactis produced nuclease). Curve Std. peanut: extract of Ara h 2 from natural source, curve rAra h 2: L. lactis produced rAra h 2, curve Sham: L. lactis produced nuclease. Error bars represent SEM of duplicate determinations.