Figure 5
From: Production of Recombinant Peanut Allergen Ara h 2 using Lactococcus lactis
Purification of rAra h 2 by hydrophic exclusion and size seperation. Culture supernatant from sample 11 applied to a hydrophic column and eluted by ethanol. 100 fractions are collected and screened. Fractions 40–48 contained the rAra h 2 as indicated by the horizontal arrow. A filtration step excluding proteins above 50 kDa and below 10 kDa resulted in a purified product (depicted as rAra h 2) as analysed by SDS-PAGE in the area of 6–100 kDa (M: Molecular weight).