Quenching solution | ATP (mM) | ADP (mM) | AMP (mM) | Total (mM) | EC |
|---|
MeOH/HEPES | 5.88 | 2.04 | 0.44 | 8.36 | 0.83 |
MeOH/AC | 4.75 | 2.11 | 1.53 | 8.39 | 0.70 |
- Intracellular concentrations of ATP, ADP and AMP in cell extracts derived from a pH-controlled continuous culture. Two samples were quenched, one with 60% MeOH and 70 mM HEPES (pH 5.5) and the other 60% MeOH and 0.85% ammonium carbonate (pH 5.5). The two cell pellets were washed with the same quenching solution, extracted with cold methanol and freeze-dried. Finally, ATP, ADP and AMP were quantified. The coefficient of variance of this quantification method was less than 7%. The intracellular concentrations were determined using the intracellular volume of E. coli (2.15 mL g-1 dry weight) [37].
- The energy charge (EC) of the cell extract was calculated by
