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Table 3 Adenine nucleotide concentrations and energy charges in cell extracts of a chemostat grown culture of Lactobacillus plantarum.

From: Comparison of quenching and extraction methodologies for metabolome analysis of Lactobacillus plantarum

Quenching solution ATP (mM) ADP (mM) AMP (mM) Total (mM) EC
MeOH/HEPES 5.88 2.04 0.44 8.36 0.83
MeOH/AC 4.75 2.11 1.53 8.39 0.70
  1. Intracellular concentrations of ATP, ADP and AMP in cell extracts derived from a pH-controlled continuous culture. Two samples were quenched, one with 60% MeOH and 70 mM HEPES (pH 5.5) and the other 60% MeOH and 0.85% ammonium carbonate (pH 5.5). The two cell pellets were washed with the same quenching solution, extracted with cold methanol and freeze-dried. Finally, ATP, ADP and AMP were quantified. The coefficient of variance of this quantification method was less than 7%. The intracellular concentrations were determined using the intracellular volume of E. coli (2.15 mL g-1 dry weight) [37].
  2. The energy charge (EC) of the cell extract was calculated by E C = [ A T P ] + 0.5 [ A D P ] [ A T P ] + [ A D P ] + [ A M P ] MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaacqWGfbqrcqWGdbWqcqGH9aqpdaWcaaqaamaadmaabaGaemyqaeKaemivaqLaemiuaafacaGLBbGaayzxaaGaey4kaSIaeGimaaJaeiOla4IaeGynauZaamWaaeaacqWGbbqqcqWGebarcqWGqbauaiaawUfacaGLDbaaaeaadaWadaqaaiabdgeabjabdsfaujabdcfaqbGaay5waiaaw2faaiabgUcaRmaadmaabaGaemyqaeKaemiraqKaemiuaafacaGLBbGaayzxaaGaey4kaSYaamWaaeaacqWGbbqqcqWGnbqtcqWGqbauaiaawUfacaGLDbaaaaaaaa@4FB9@