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Table 1 Primers and probes used in this work.

From: Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction

  Sequence 5' → 3' Position Tm °C Th °C
AOX1     
Forward primer AGC AGG TGA GAA CAA CCT CAA C 583 66  
Reverse primer AAG GTC CAC CGT AGG CAT TAG A 1988 66  
Detection ACC ATT GGC GTA CCA TTG 1510 54 55
Capture GAA TCT CAG CAT CAC CAC 1475 54 55
Helper GAC TCG TAC TGA GGC TTG 1440 56 55
26S rRNA     
Forward primer AAT GAG TGG TGA CAC GAG AC 8 60  
Reverse primer GGA TGT CGG AGA TGC GTA G 168 62  
Detection GCG TAG ACG CAC CAA CT 155 54 50
Capture ACT CCT CCT ACG CCT TC 61 54 50
Helper TTG GCC GTC TCG TGT CA 33 54 50
PDI     
Forward primer TGT CCG CTC TCA CAC TAG CA 726 62  
Reverse primer TTC CTC GAC TGG TCT GAG TG 2182 62  
Detection CCG ACT AGC TTG AAG ACT 1827 54 55
Capture CCT CTC CTG CGA TGA ATT 1760 54 55
Helper TCT CAA TCA GCT CGG TCA 1742 54 55
FLD1     
Forward primer TCA CTG GTG TAT GCC ACA CT 839 60  
Reverse primer GAT GTT GTC CAG GTC ATG TC 1788 60  
Detection AGC AGT GTT GAT AGC AGC AC 1278 60 55
Capture T AG GAG CTT CGT CTT GGA CT 1213 60 55
Helper TAA CCA CT GAGA TGT CAG CC 1192 60 55
KAR2     
Forward primer GCC ATG CTA TTG GTC GTA GT 49 60  
Reverse primer GGT AGG CTC GTT CAC AAT TC 633 60  
Detection TTG AAG TAG GCT GGA ACG GT 554 60 50
Capture GTG GCT TGA CGT TGA GCG T 575 60 50
Helper CGA TGA GAC CGG CAT CCT T 595 60 50
ROL     
Forward primer CTG ATG GTG GTA AGG TTG T 1271 56  
Reverse primer AAG GAA CGA TAG AGT TAC TG 2020 56  
Detection TGC ACC ACC GAG TGA GTG A 1716 60 50
Capture AGA TCC ATA CCG GCA AGC AA 1742 60 50
Helper ACA GTC TTG GTT CAC GTT GG 1765 60 50
  1. Tm: melting temperature. Th: hybridisation temperature. Position: First nucleotide localization within the corresponding gene sequence at GeneBank