Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction

Table 1 Primers and probes used in this work.

	Sequence 5' → 3'	Position	Tm °C	Th °C
*AOX1*
Forward primer	AGC AGG TGA GAA CAA CCT CAA C	583	66
Reverse primer	AAG GTC CAC CGT AGG CAT TAG A	1988	66
Detection	ACC ATT GGC GTA CCA TTG	1510	54	55
Capture	GAA TCT CAG CAT CAC CAC	1475	54	55
Helper	GAC TCG TAC TGA GGC TTG	1440	56	55
*26S rRNA*
Forward primer	AAT GAG TGG TGA CAC GAG AC	8	60
Reverse primer	GGA TGT CGG AGA TGC GTA G	168	62
Detection	GCG TAG ACG CAC CAA CT	155	54	50
Capture	ACT CCT CCT ACG CCT TC	61	54	50
Helper	TTG GCC GTC TCG TGT CA	33	54	50
*PDI*
Forward primer	TGT CCG CTC TCA CAC TAG CA	726	62
Reverse primer	TTC CTC GAC TGG TCT GAG TG	2182	62
Detection	CCG ACT AGC TTG AAG ACT	1827	54	55
Capture	CCT CTC CTG CGA TGA ATT	1760	54	55
Helper	TCT CAA TCA GCT CGG TCA	1742	54	55
*FLD1*
Forward primer	TCA CTG GTG TAT GCC ACA CT	839	60
Reverse primer	GAT GTT GTC CAG GTC ATG TC	1788	60
Detection	AGC AGT GTT GAT AGC AGC AC	1278	60	55
Capture	T AG GAG CTT CGT CTT GGA CT	1213	60	55
Helper	TAA CCA CT GAGA TGT CAG CC	1192	60	55
*KAR2*
Forward primer	GCC ATG CTA TTG GTC GTA GT	49	60
Reverse primer	GGT AGG CTC GTT CAC AAT TC	633	60
Detection	TTG AAG TAG GCT GGA ACG GT	554	60	50
Capture	GTG GCT TGA CGT TGA GCG T	575	60	50
Helper	CGA TGA GAC CGG CAT CCT T	595	60	50
*ROL*
Forward primer	CTG ATG GTG GTA AGG TTG T	1271	56
Reverse primer	AAG GAA CGA TAG AGT TAC TG	2020	56
Detection	TGC ACC ACC GAG TGA GTG A	1716	60	50
Capture	AGA TCC ATA CCG GCA AGC AA	1742	60	50
Helper	ACA GTC TTG GTT CAC GTT GG	1765	60	50

Tm: melting temperature. Th: hybridisation temperature. Position: First nucleotide localization within the corresponding gene sequence at GeneBank

ISSN: 1475-2859