Transcriptional analysis in shake flask cultures of the P. pastoris X-33 pROL. Transcriptional levels of AOX1, FLD1, PDI, ROL and KAR2 expressed as fmol of mRNA per μg of total RNA. Time = 0 hours corresponds to change of cells from BMG medium to the induction medium. (A) Cells grown on BMG and BMG+DTT media; (B) Cells grown on BMM and BMS media. (C) Control cultivation: X-33 cells grown on BMM and BMS media.