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Table 1 Plasmids used in this study.

From: Recombinant production of Streptococcus equisimilis streptokinase by Streptomyces lividans

Name Relevant properties Source or reference
pGEM-SK pGEM®-T Easy derivative containing the Streptococcus equisimilis skc-2 gene This work
pBS-CBSS pBluescript KS(+) derivative containing the Streptomyces venezuelae vsi promoter and part of the mature vsi gene [16]
pBSVX pBluescript KS(+) derivative containing the Streptomcyes venezuelae vsi promoter and the signal sequence of Streptomyces lividans xlnC [17]
pBSVXM pBSVX derivative containing a unique Eco RI site downstream of the signal sequence of Streptomyces lividans xlnC This work
pUWL-218 Escherichia coli-Streptomyces shuttle vector, multiple cloning site, ApR, TsrR [40]
pOW15 pUWL-218 derivative E. coli-Streptomyces shuttle vector containing the oriT fragment for interspecies DNA conjugation. Rosabal et al., unpublished.
pOVsiSK pOW15 derivative containing the Streptomyces venezuelae vsi promoter, and signal sequence, and the Streptococcus equisimilis skc-2 gene This work
pOXlnCSK pOW15 derivative containing the Streptomyces venezuelae vsi promoter, the Streptomyces lividans xlnC signal sequence and the Streptococcus equisimilis skc-2 gene This work