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Table 1 Plasmids used in this study.

From: Recombinant production of Streptococcus equisimilis streptokinase by Streptomyces lividans

Name

Relevant properties

Source or reference

pGEM-SK

pGEM®-T Easy derivative containing the Streptococcus equisimilis skc-2 gene

This work

pBS-CBSS

pBluescript KS(+) derivative containing the Streptomyces venezuelae vsi promoter and part of the mature vsi gene

[16]

pBSVX

pBluescript KS(+) derivative containing the Streptomcyes venezuelae vsi promoter and the signal sequence of Streptomyces lividans xlnC

[17]

pBSVXM

pBSVX derivative containing a unique Eco RI site downstream of the signal sequence of Streptomyces lividans xlnC

This work

pUWL-218

Escherichia coli-Streptomyces shuttle vector, multiple cloning site, ApR, TsrR

[40]

pOW15

pUWL-218 derivative E. coli-Streptomyces shuttle vector containing the oriT fragment for interspecies DNA conjugation.

Rosabal et al., unpublished.

pOVsiSK

pOW15 derivative containing the Streptomyces venezuelae vsi promoter, and signal sequence, and the Streptococcus equisimilis skc-2 gene

This work

pOXlnCSK

pOW15 derivative containing the Streptomyces venezuelae vsi promoter, the Streptomyces lividans xlnC signal sequence and the Streptococcus equisimilis skc-2 gene

This work