From: Recombinant production of Streptococcus equisimilis streptokinase by Streptomyces lividans
Name | Relevant properties | Source or reference |
---|---|---|
pGEM-SK | pGEM®-T Easy derivative containing the Streptococcus equisimilis skc-2 gene | This work |
pBS-CBSS | pBluescript KS(+) derivative containing the Streptomyces venezuelae vsi promoter and part of the mature vsi gene | [16] |
pBSVX | pBluescript KS(+) derivative containing the Streptomcyes venezuelae vsi promoter and the signal sequence of Streptomyces lividans xlnC | [17] |
pBSVXM | pBSVX derivative containing a unique Eco RI site downstream of the signal sequence of Streptomyces lividans xlnC | This work |
pUWL-218 | Escherichia coli-Streptomyces shuttle vector, multiple cloning site, ApR, TsrR | [40] |
pOW15 | pUWL-218 derivative E. coli-Streptomyces shuttle vector containing the oriT fragment for interspecies DNA conjugation. | Rosabal et al., unpublished. |
pOVsiSK | pOW15 derivative containing the Streptomyces venezuelae vsi promoter, and signal sequence, and the Streptococcus equisimilis skc-2 gene | This work |
pOXlnCSK | pOW15 derivative containing the Streptomyces venezuelae vsi promoter, the Streptomyces lividans xlnC signal sequence and the Streptococcus equisimilis skc-2 gene | This work |