Figure 3
From: Recombinant production of Streptococcus equisimilis streptokinase by Streptomyces lividans
Purification of extracellular SK from S. lividans culture supernatants upon small-scale fermentation. (A) 10% SDS-PAGE stained with Coomassie blue R-250, and (B) Immunoblotting analysis using a monoclonal anti-SK antibody. Lane 1, 25 μg of crude extract of S. lividans TK24 [pOW15]; lane 2, 25 μg of material precipitated with (NH4)2SO4 of S. lividans TK24 [pOW15]; lane 3, 25 μg of crude extract of S. lividans TK24 [pOVsiSK]; lane 4, 25 μg of proteins precipitated with (NH4)2SO4 of S. lividans TK24 [pOVsiSK]; lane 5, 25 μg of pooled anion exchange chromatography protein fractions with 58% purity; lane 6, 1 μg of SK standard; lane 7, Broad-range protein molecular weight markers.