Figure 1From: Production of recombinant antibody fragments in Bacillus megateriumConstruction of plasmid pEJBmD1.3scFv for the production of scFv antibody fragment D1.3 in B. megaterium. The complete scFv ORF plus the complete promoter region were verified by nucleotide sequencing. Abbreviations: bla: β-lactamase gene for ampicillin resistence; colE1: E. coli origin of plasmid replication; F1 IR: intergenic region of phage f1; gIII: fd phage gene III; His-tag: synthetic tag binding to 6xhistidine; ori: B. megaterium origin of plasmid replication; P lacZ : promoter of the bacterial lac operon; P xylA : xylose inducible promoter; RBS: ribosome binding site; repU: a gene essential for plasmid replication in B. megaterium; scFv: single chain fragment variable; SPlipA: signal peptide sequence of B. megaterium extracellular esterase LipA; SPpelB: signal peptide sequence of bacterial pectate lyase; strep-tag II: synthetic tag binding to streptactin; terminator: sequence terminating transcription; tet: tetracyclin resistence gene; VH: sequence encoding the variable fragment of the heavy chain; VL: sequence encoding the variable fragment of the light chain chain; xylR: xylose repressorBack to article page