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Figure 1 | Microbial Cell Factories

Figure 1

From: Production of recombinant antibody fragments in Bacillus megaterium

Figure 1

Construction of plasmid pEJBmD1.3scFv for the production of scFv antibody fragment D1.3 in B. megaterium. The complete scFv ORF plus the complete promoter region were verified by nucleotide sequencing. Abbreviations: bla: β-lactamase gene for ampicillin resistence; colE1: E. coli origin of plasmid replication; F1 IR: intergenic region of phage f1; gIII: fd phage gene III; His-tag: synthetic tag binding to 6xhistidine; ori: B. megaterium origin of plasmid replication; P lacZ : promoter of the bacterial lac operon; P xylA : xylose inducible promoter; RBS: ribosome binding site; repU: a gene essential for plasmid replication in B. megaterium; scFv: single chain fragment variable; SPlipA: signal peptide sequence of B. megaterium extracellular esterase LipA; SPpelB: signal peptide sequence of bacterial pectate lyase; strep-tag II: synthetic tag binding to streptactin; terminator: sequence terminating transcription; tet: tetracyclin resistence gene; VH: sequence encoding the variable fragment of the heavy chain; VL: sequence encoding the variable fragment of the light chain chain; xylR: xylose repressor

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