Microbial Cell Factories

Table 5 Measured values of amino acids by NMR analysis in growth phase of C. glutamicum.

From: Study on roles of anaplerotic pathways in glutamate overproduction of Corynebacterium glutamicum by metabolic flux analysis

Amino Acid	Pre.		I _s	I _d1	I _d2	I _dd	I _t
Ser C3	GAP C3	measured	0.37	0.63
		estimated	0.35	0.65
Gly C2	GAP C2	measured	0.17	0.83
		estimated	0.18	0.82
Ala C2	Pyr C2	measured	0.09	0.06	0.13	0.72
		estimated	0.11	0.06	0.11	0.72
Ala C3	Pyr C3	measured	0.33	0.67
		estimated	0.34	0.66
Val C4	Pyr C3	measured	0.33	0.67
		estimated	0.34	0.66
Leu C5	Pyr C3	measured	0.33	0.67
		estimated	0.34	0.66
Asp C3	Oxa C3	measured	0.31	0.20	0.28	0.21
		estimated	0.31	0.21	0.29	0.19
Thr C3	Oxa C3	measured	0.36	0.54			0.11
		estimated	0.40	0.51			0.09
Thr C4	Oxa C4	measured	0.42	0.58
		estimated	0.38	0.62
Glu C3	αKG C3	measured	0.43	0.47			0.10
		estimated	0.44	0.45			0.11
Arg C5	αKG C5	measured	0.18	0.82
		estimated	0.17	0.83

Symbols: I_s, I_d1, I_d2, I_dd, and I_t indicate coupling pattern of NMR, that is, singlet, doublet1, doublet2, double-doublet, and triplet, respectively. Abbreviations: Pre., precursor of amino acid. For others, see Fig. 1.

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