Microbial Cell Factories

Table 4 Mass isotopomer distributions of amino acids in glutamate production phase of C. glutamicum, where glutamate flux was 68 with respect to glucose uptake of 100.

From: Study on roles of anaplerotic pathways in glutamate overproduction of Corynebacterium glutamicum by metabolic flux analysis

		m 0	M 1	m 2	m 3	m 4	m 5
Ala-57	measured	0.676	0.145	0.025	0.154
	estimated	0.670	0.140	0.042	0.148
Gly-57	measured	0.768	0.073	0.158
	estimated	0.769	0.072	0.159
Val-57	measured	0.463	0.189	0.164	0.133	0.021	0.030
	estimated	0.472	0.179	0.164	0.134	0.025	0.026
Ser-57	measured	0.651	0.170	0.082	0.097
	estimated	0.657	0.163	0.087	0.093
Phe-57	measured	0.324	0.160	0.150	0.150	0.104	0.051
	estimated	0.334	0.169	0.139	0.141	0.104	0.055
Asp-57	measured	0.586	0.185	0.102	0.116	0.010
	estimated	0.582	0.183	0.104	0.117	0.014
Glu-57	measured	0.435	0.211	0.227	0.091	0.028	0.009
	estimated	0.436	0.207	0.229	0.088	0.031	0.010
His-57	measured	0.477	0.223	0.099	0.073	0.032	0.080
	estimated	0.466	0.232	0.099	0.072	0.047	0.071
Val-159	measured	0.484	0.162	0.272	0.050	0.031
	estimated	0.495	0.168	0.263	0.042	0.031
Leu-159	measured	0.346	0.256	0.246	0.110	0.032	0.010
	estimated	0.359	0.258	0.237	0.103	0.034	0.009
Ile-159	measured	0.446	0.201	0.228	0.086	0.033	0.007
	estimated	0.436	0.207	0.229	0.088	0.031	0.010
Ser-159	measured	0.693	0.206	0.101
	estimated	0.689	0.203	0.108
Phe-159	measured	0.351	0.164	0.212	0.084	0.105	0.040
	estimated	0.350	0.162	0.214	0.084	0.105	0.037

The underlined values are those used for ¹³C MFA based on the threshold (value > 0.06, error < 3%).-57 and -159 in amino acids indicate [M-57] and [M-159] groups of GC-MS analysis, whose ion fragments have high intensities [26, 42, 43].

Back to article page

ISSN: 1475-2859

Contact us

Submission enquiries: journalsubmissions@springernature.com