Microbial Cell Factories

Table 3 Mass isotopomer distributions of amino acids in glutamate production phase of C. glutamicum, where glutamate flux was 20 with respect to glucose uptake of 100.

From: Study on roles of anaplerotic pathways in glutamate overproduction of Corynebacterium glutamicum by metabolic flux analysis

		m 0	m 1	m 2	m 3	m 4	m 5
Ala-57	measured	0.702	0.129	0.047	0.122
	estimated	0.706	0.135	0.041	0.117
Gly-57	measured	0.782	0.087	0.131
	estimated	0.790	0.083	0.127
Val-57	measured	0.529	0.166	0.139	0.111	0.029	0.026
	estimated	0.527	0.178	0.148	0.111	0.019	0.017
Ser-57	measured	0.701	0.139	0.058	0.102
	estimated	0.708	0.139	0.058	0.095
Phe-57	measured	0.404	0.167	0.123	0.121	0.082	0.048
	estimated	0.381	0.181	0.135	0.135	0.088	0.041
Asp-57	measured	0.572	0.215	0.117	0.077	0.019
	estimated	0.575	0.211	0.130	0.075	0.008
Glu-57	measured	0.466	0.226	0.196	0.077	0.028	0.007
	estimated	0.462	0.236	0.202	0.073	0.021	0.005
His-57	measured	0.487	0.199	0.117	0.100	0.048	0.036
	estimated	0.492	0.228	0.112	0.082	0.035	0.045
Val-159	measured	0.550	0.164	0.210	0.050	0.026
	estimated	0.557	0.163	0.227	0.032	0.021
Leu-159	measured	0.452	0.231	0.198	0.083	0.028	0.008
	estimated	0.430	0.253	0.213	0.076	0.023	0.005
Ile-159	measured	0.471	0.225	0.194	0.076	0.026	0.008
	estimated	0.462	0.236	0.202	0.073	0.021	0.005
Ser-159	measured	0.721	0.158	0.121
	estimated	0.747	0.138	0.115
Phe-159	measured	0.418	0.169	0.167	0.092	0.077	0.037
	estimated	0.402	0.170	0.200	0.085	0.085	0.029

The underlined values are those used for ¹³C MFA based on the threshold (value > 0.06, error < 3%).-57 and -159 in amino acids indicate [M-57] and [M-159] groups of GC-MS analysis, whose ion fragments have high intensities [26, 42, 43].

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ISSN: 1475-2859

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