Microbial Cell Factories

Table 2 Mass isotopomer distributions of amino acids in growth phase of C. glutamicum.

From: Study on roles of anaplerotic pathways in glutamate overproduction of Corynebacterium glutamicum by metabolic flux analysis

		m 0	m 1	m 2	m 3	m 4	m 5
Ala-57	measured	0.677	0.128	0.040	0.155
	estimated	0.665	0.138	0.041	0.156
Gly-57	measured	0.753	0.076	0.171
	estimated	0.754	0.076	0.170
Val-57	measured	0.472	0.162	0.158	0.138	0.035	0.034
	estimated	0.465	0.178	0.164	0.139	0.026	0.028
Ser-57	measured	0.654	0.154	0.047	0.146
	estimated	0.669	0.136	0.047	0.147
Phe-57	measured	0.310	0.165	0.135	0.151	0.103	0.061
	estimated	0.308	0.174	0.141	0.153	0.106	0.057
Asp-57	measured	0.495	0.237	0.128	0.106	0.033
	estimated	0.506	0.227	0.155	0.099	0.013
Glu-57	measured	0.389	0.226	0.221	0.111	0.039	0.013
	estimated	0.386	0.243	0.230	0.099	0.032	0.009
His-57	measured	0.411	0.209	0.130	0.103	0.058	0.058
	estimated	0.422	0.243	0.123	0.097	0.053	0.054
Val-159	measured	0.496	0.156	0.258	0.054	0.036
	estimated	0.489	0.170	0.265	0.044	0.032
Leu-159	measured	0.361	0.243	0.239	0.105	0.040	0.012
	estimated	0.357	0.256	0.240	0.103	0.035	0.009
Ile-159	measured	0.398	0.224	0.229	0.099	0.036	0.013
	estimated	0.386	0.243	0.230	0.099	0.032	0.009
Ser-159	measured	0.691	0.131	0.178
	estimated	0.702	0.131	0.166
Phe-159	measured	0.350	0.157	0.194	0.109	0.093	0.046
	estimated	0.323	0.170	0.212	0.103	0.103	0.042

The underlined values are those used for ¹³C MFA based on the threshold (value > 0.06, error < 3%). -57 and -159 in amino acids indicate [M-57] and [M-159] groups of GC-MS analysis, whose ion fragments have high intensities [26, 42, 43].

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