Figure 4

Comparison of fluxes in anaplerotic pathways in different phases. Black, white, zebra, and dotted bars indicate the reactions catalyzed by Pc, PEPc, PEPck, and malic enzymes, respectively. Phases 1 and 2 indicate the fermentations with the glutamate production fluxes of 20 and 68, respectively. Flux values of the anaplerosis in three phases (growth, Phase 1, and Phase 2) were as follows: 0, 10, 56 by Pc, 37, 41, 39 by PEPc, 4, 0, 6 by PEPck, and 13, 18, 18 by malic enzymes, respectively. Error bars indicate those with fluxes induced by the errors of GC-MS data, which were obtained from 20 sets of artificial GC-MS data with a variance due to the observed experimental errors (below 2% in this study), as previously described [25].