Figure 3
From: Homologous high-throughput expression and purification of highly conserved E coli proteins
Step-by-step efficiency of cloning, small-scale protein expression and purification of ECHH proteins. 43 purified proteins with high and moderate yields were subjected to large-scale expression. 34 proteins could be purified with a minimum of 500 μg. Two target proteins were subjected to Gateway recombination cloning in order to express fusion proteins with MBP-His7, GST-His7, and NusA-His6, respectively. Both proteins fused to MBP-His7 were purified with a minimum of 500 μg.