Rivoflavin may interfere with on-line monitoring of secreted green fluorescence protein fusion proteins in Pichia pastoris

Table 1 Primers used for SOE-PCR

Name	Sequence	Tm °C
DR2	gtagagcggccgccaaacagcttccttcgttgatatcaaagtaactca	55.8
DR3	aaattcaccagaaccagcagaaccagcagaacc tttgtatagttcatccatgccatgtgtaatccc	55.8
DR4	ccaagacgaccaagacgaccaagaccacttaaa tctgatggtggtaaggttgttgctgctactactg	59.3
DR6	aaattcaccagaaccagcagaaccagcagaacc caaacagcttccttcgttgatatca	56.5
DR7	ggttctgctggttctgctggttctggtgaattt agtaaaggagaagaacttttcactggagt	53.0
DR9	ctctcgagaaaagagaggctgaagctgaattcatgagtaaaggagaagaacttttca	55.5
DR10	gtatctctcgagaaaagagaggctgaagctagtaaaggagaagaactt	55.5
DR11	gcggccgcttattacaaacagcttccttcgttgatatca	56.5
DR12	gtatctctcgagaaaagagaggctgaagcttctgatggtggtaaggtt	56.5
DR13	gcggccgcttattatttgtatagttcatccatgccatgtg	56.8
DR14	gtatctctcgagaaaagagaggctgaagct	61.3
DR15	gcggccgcttattatttgtatagttcatc	61.2

ISSN: 1475-2859