Figure 2

Bioreactor batch culture of clone GFP-ROL 1.5.5. Bioreactor batch cultivation of P. pastoris strain expressing the GFP-ROL fusion protein under the control of the FLD1 promoter. Sorbitol was used as the carbon source while methylamine was used both as the nitrogen source and protein inducer. Biomass (●), sorbitol concentration (□) and lipase activity (△) are depicted. Arrow indicates the addition of 15 g·L-¹ of sorbitol.