Figure 1From: Rivoflavin may interfere with on-line monitoring of secreted green fluorescence protein fusion proteins in Pichia pastorisWestern blot analysis of shake flask and bioreactor batch cultivation samples. Western blots using anti-ROL antibodies (A) and anti-GFP antibodies (B). Lane 1: ROL positive control, lane 2: culture supernatant from clone GFP-ROL 1.5.5; lane 3: culture supernatant from clone ROL-GFP 2.15.2; lane 4: culture supernatant from bioreactor culture of clone GFP-ROL 1.5.5.Back to article page