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Table 3 Quantitative assays of BLG in different fractions of BL23(int:nisRK):LEISS-Nuc-BLG

From: Efficient production and secretion of bovine β-lactoglobulin by Lactobacillus casei

Fractionb

Concentration (ng BLG/mg total protein)a

 

Protocol Ac

Protocol Bc

Cs (% BLGn)d

725,8 ± 33,7 (8%)

2481,6 ± 407,2 (1%)

Ci

6573,6 ± 631,6

884,7 ± 283,4

Total

7299.4 ± 597.9

3366.8 ± 356.4

  1. a Values represent the means ± SD of at least two independent experiments; b Cs, soluble cytoplasmic fraction; Ci, insoluble cytoplasmic fraction; c see nisin induction in Materials and Methods; d Percentage of BLG in its native form.
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Microbial Cell Factories

ISSN: 1475-2859

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