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Table 3 Quantitative assays of BLG in different fractions of BL23(int:nisRK):LEISS-Nuc-BLG

From: Efficient production and secretion of bovine β-lactoglobulin by Lactobacillus casei

Fractionb Concentration (ng BLG/mg total protein)a
  Protocol Ac Protocol Bc
Cs (% BLGn)d 725,8 ± 33,7 (8%) 2481,6 ± 407,2 (1%)
Ci 6573,6 ± 631,6 884,7 ± 283,4
Total 7299.4 ± 597.9 3366.8 ± 356.4
  1. a Values represent the means ± SD of at least two independent experiments; b Cs, soluble cytoplasmic fraction; Ci, insoluble cytoplasmic fraction; c see nisin induction in Materials and Methods; d Percentage of BLG in its native form.