Table 2 Quantitative assays of BLG in different fractions of recombinant L. casei strains
From: Efficient production and secretion of bovine β-lactoglobulin by Lactobacillus casei
Fractionb | Concentration of BLG (μg/L)a | |||||
|---|---|---|---|---|---|---|
BL23 + pNZ9520c | BL23(int:nisRK)d | |||||
pSEC-Nuc | pCYT:BLG | pSEC:BLG | pSEC:LEISS-BLG | pSEC:LEISS-Nuc-BLG | pSEC:LEISS-Nuc-BLG | |
S (% BLGn)e | ndf | 1.4 ± 2.2 (43%) | 1.4 ± 1.8 (0%) | 8.7 ± 2.3 (40%) | 27 ± 3.7 (46%) | 29.4 ± 6.4 (46%) |
Cs (% BLGn) | nd | 57.6 ± 10.3 (27%) | 57.6 ± 27.3 (1%) | 38.4 ± 12 (1%) | 44.1 ± 12 (8%) | 57.7 ± 6.3 (8%) |
Ci | nd | 19.2 ± 2.6 | 647.4 ± 301.1 | 975 ± 238.1 | 342.9 ± 128.7 | 526.9 ± 131.4 |
Total | nd | 78.2 ± 6.5 | 706.4 ± 303.9 | 1022.1 ± 327.5 | 413.9 ± 116 | 613.9 ± 144.1 |