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Table 2 Quantitative assays of BLG in different fractions of recombinant L. casei strains

From: Efficient production and secretion of bovine β-lactoglobulin by Lactobacillus casei

Fractionb Concentration of BLG (μg/L)a
  BL23 + pNZ9520c BL23(int:nisRK)d
  pSEC-Nuc pCYT:BLG pSEC:BLG pSEC:LEISS-BLG pSEC:LEISS-Nuc-BLG pSEC:LEISS-Nuc-BLG
S (% BLGn)e ndf 1.4 ± 2.2 (43%) 1.4 ± 1.8 (0%) 8.7 ± 2.3 (40%) 27 ± 3.7 (46%) 29.4 ± 6.4 (46%)
Cs (% BLGn) nd 57.6 ± 10.3 (27%) 57.6 ± 27.3 (1%) 38.4 ± 12 (1%) 44.1 ± 12 (8%) 57.7 ± 6.3 (8%)
Ci nd 19.2 ± 2.6 647.4 ± 301.1 975 ± 238.1 342.9 ± 128.7 526.9 ± 131.4
Total nd 78.2 ± 6.5 706.4 ± 303.9 1022.1 ± 327.5 413.9 ± 116 613.9 ± 144.1
  1. a Values represent the means ± SD of at least three independent experiments; b S, supernatant; Cs, soluble cytoplasmic fraction; Ci, insoluble cytoplasmic fraction; c L. casei BL23 cells transformed with pNZ9520 plasmid; d L. casei BL23 cells with nisRK genes integrated in the chromosome; e Percentage of BLG in its native form; f nd, not detectable.