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Table 1 Bacterial strains and plasmids

From: Efficient production and secretion of bovine β-lactoglobulin by Lactobacillus casei

Strain or plasmid Characteristics Reference
L. casei   
   BL23 L. casei ATCC 393 (pLZ15-) [26]
   BL23(int:nisRK) BL23 containing the nisRK genes integrated to the tRNASer locus; obtained by transformation with pMEC10 This work
Plasmids   
   pVE3655 Cmr, ori(pWV01), carries the nisin-inducible promoter P nisA  
   pCYT:BLG Cmr, ori(pWV01), with a DNA fragment encoding the BLG mature moiety expressed under PnisA transcriptional control [6]
   pSEC:BLG Cmr, ori(pWV01), with a DNA fragment encoding the precursor SPUsp45-BLG expressed under PnisA transcriptional control [6]
   pSEC:LEISS-BLG Cmr, ori(pWV01), with a DNA fragment encoding the precursor SPUsp45-LEISS-BLG expressed under PnisA transcriptional control [8]
   pSEC:LEISS-Nuc-BLG Cmr, ori(pWV01), with a DNA fragment encoding the precursor SPUsp45-LEISS-Nuc-BLG expressed under PnisA transcriptional control [8]
   pSEC:Nuc Cmr, ori(pWV01), with a DNA fragment encoding the precursor SPUsp45-Nuc expressed under under PnisA transcriptional control [18]
   pNZ9520 Emr, nisRK cloned in pIL253 [17]
   pMEC10 Emr, integration plasmid containing the nisRK genes and the int-attP cassette for integration to the tRNASer locus. [21]