Efficient production and secretion of bovine β-lactoglobulin by Lactobacillus casei

Table 1 Bacterial strains and plasmids

Strain or plasmid	Characteristics	Reference
L. casei
BL23	L. casei ATCC 393 (pLZ15^-)	[26]
BL23(int:nisRK)	BL23 containing the nisRK genes integrated to the tRNA^Ser locus; obtained by transformation with pMEC10	This work
Plasmids
pVE3655	Cm^r, ori(pWV01), carries the nisin-inducible promoter P_nisA
pCYT:BLG	Cm^r, ori(pWV01), with a DNA fragment encoding the BLG mature moiety expressed under P_nisA transcriptional control	[6]
pSEC:BLG	Cm^r, ori(pWV01), with a DNA fragment encoding the precursor SP_Usp45-BLG expressed under P_nisA transcriptional control	[6]
pSEC:LEISS-BLG	Cm^r, ori(pWV01), with a DNA fragment encoding the precursor SP_Usp45-LEISS-BLG expressed under P_nisA transcriptional control	[8]
pSEC:LEISS-Nuc-BLG	Cm^r, ori(pWV01), with a DNA fragment encoding the precursor SP_Usp45-LEISS-Nuc-BLG expressed under P_nisA transcriptional control	[8]
pSEC:Nuc	Cm^r, ori(pWV01), with a DNA fragment encoding the precursor SP_Usp45-Nuc expressed under under P_nisA transcriptional control	[18]
pNZ9520	Em^r, nisRK cloned in pIL253	[17]
pMEC10	Em^r, integration plasmid containing the nisRK genes and the int-attP cassette for integration to the tRNA^Ser locus.	[21]

ISSN: 1475-2859