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Figure 4 | Microbial Cell Factories

Figure 4

From: Evidence for glycosylation on a DNA-binding protein of Salmonella enterica

Figure 4

Total carbohydrate analysis of Dps by GC-MS. J-Dps (200 picomoles) was hydrolyzed with methanolic 0.5 N HCl for 4h, at 85°C, re-N-acetylated and analyzed by GC-MS as trimethylsilyl (TMS) derivatives. s-Ins, scyllo-inositol (internal standard, 1 nmol); Std., carbohydrate standard mixture containing 500 pmol of each of the sugars Rha, Fuc, Xyl, Man, Gal, and Glc, and 1 nmol of each of the sugars GalNAc, GlcNAc, and NeuAc (NANA). Subscript numbers indicate Fuc, Xyl, Man, Gal, and Glc isomers. The 1-O-methyl-Manp-TMS4 derivative in the Dps sample was confirmed after electron impact fragmentation. Characteristic ions at m/z 73, 133, 147, 204, and 128, similar in intensity to those obtained from the fragmentation of an authentic 1-O-methyl-Man p-TMS4 standard, were observed. The unknown ion species (?) could not be identified through database matching of the electron impact fragmentation spectrum.

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