Table 1 Purification of recombinant glucose dehydrogenase from H. mediterranei
From: An optimized method to produce halophilic proteins in Escherichia coli
Enzyme fraction | Vol. (ml) | UT | mgT | Specific activity (U/mg) | Enrichment (-fold) | Yield (%) | |
|---|---|---|---|---|---|---|---|
Inclusion bodies refolding in 20 mM Tris-HCl pH 7.4, 2 M NaCl, 1 mM EDTA | 500 | 2210 | 20.9 | 106 | 1.0 | 100 | |
(NH 4 ) 2 SO 4 Precipitation | Supernatant | 500 | 2180 | 18.7 | 117 | 1.1 | 99 |
Pellet | 20 | 27 | 1.7 | 16 | ---- | ---- | |
DEAE-cellulose | 18 | 1666 | 8.9 | 187 | 1.8 | 75 |