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Table 1 Purification of recombinant glucose dehydrogenase from H. mediterranei

From: An optimized method to produce halophilic proteins in Escherichia coli

Enzyme fraction   Vol. (ml) UT mgT Specific activity (U/mg) Enrichment (-fold) Yield (%)
Inclusion bodies refolding in 20 mM Tris-HCl pH 7.4, 2 M NaCl, 1 mM EDTA   500 2210 20.9 106 1.0 100
(NH 4 ) 2 SO 4 Precipitation Supernatant 500 2180 18.7 117 1.1 99
  Pellet 20 27 1.7 16 ---- ----
DEAE-cellulose   18 1666 8.9 187 1.8 75