Figure 2
From: Purification of a chimeric virus-like particle from a complex culture medium
Silver Staining of a denaturing 12% poliacrilamide gel presenting the protein pattern during centration/dialysis in a 300 kDa ultrafiltration membrane. Western blot were performed using a conjugated antibody, anti-HA HRP Roche). Molecular Weight markers were marked in kDa. Lane A. Supernatant of transfected 293T prior to concentration B. First permeate C. Second permeate D. Third permeate E. Fourth permeate F. Fifth permeate G. Sixth permeate H. Concentrated supernatant.