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Table 2 Retention times of heterodimeric hFSH before dissociation, of α-and β-subunits after dissociation and relative retention times (tRR) of the α and β subunits with basis on heterodimeric hFSH, determined on RP-HPLC (n = 2).

From: Analysis and characterization of different preparations of recombinant human follicle stimulating hormone (hFSH) and of its subunits

SAMPLE

heterodimer tR

β-subunit tR

α-subunit tR

β-subunit tRRa

α-subunit tRRa

p-hFSH

24.43 ± 0.156

26.98 ± 0.160

36.63 ± 0.198

1.104

1.499

r-hFSH Gonal F

25.19 ± 0.129

27.62 ± 0.235

38.86 ± 0.214

1.096

1.543

r-hFSH Puregon

25.29 ± 0.070

27.85 ± 0.131

38.16 ± 0.127

1.101

1.509

  1. atRR, (relative retention time) = tRsubunit/tRheterodimer