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Table 2 Retention times of heterodimeric hFSH before dissociation, of α-and β-subunits after dissociation and relative retention times (tRR) of the α and β subunits with basis on heterodimeric hFSH, determined on RP-HPLC (n = 2).

From: Analysis and characterization of different preparations of recombinant human follicle stimulating hormone (hFSH) and of its subunits

SAMPLE heterodimer tR β-subunit tR α-subunit tR β-subunit tRRa α-subunit tRRa
p-hFSH 24.43 ± 0.156 26.98 ± 0.160 36.63 ± 0.198 1.104 1.499
r-hFSH Gonal F 25.19 ± 0.129 27.62 ± 0.235 38.86 ± 0.214 1.096 1.543
r-hFSH Puregon 25.29 ± 0.070 27.85 ± 0.131 38.16 ± 0.127 1.101 1.509
  1. atRR, (relative retention time) = tRsubunit/tRheterodimer