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Table 1 :

From: Monitoring protein expression levels in E. coli using a high throughput approach

  Expression strain Culture medium Lysis and purification buffer
Condition 1 Strain 2 Medium 2 Buffer 1
Condition 2 Strain 3 Medium 1 Buffer 4
Condition 3 Strain 3 Medium 2 Buffer 2
Condition 4 Strain 1 Medium 1 Buffer 1
Condition 5 Strain 2 Medium 3 Buffer 3
Condition 6 Strain 1 Medium 3 Buffer 4
Condition 7 Strain 2 Medium 2 Buffer 4
Condition 8 Strain 1 Medium 2 Buffer 3
Condition 9 Strain 3 Medium 2 Buffer 5
Condition 10 Strain 1 Medium 1 Buffer 5
Condition 11 Strain 2 Medium 3 Buffer 5
Condition 12 Strain 3 Medium 1 Buffer 3
Condition 13 Strain 3 Medium 3 Buffer 1
Condition 14 Strain 2 Medium 1 Buffer 2
Condition 15 Strain 1 Medium 3 Buffer 2
  1. Sparse matrix design (http://www.igs.cnrs-mrs.fr/samba/samba.html): Strain 1: BL21(DE3)/Strain 2: C43(DE3)(from OverExpress™)/Strain3: BL21(DE3) harboring pG-KJE8 vector from Takara Medium 1: LB/Medium 2: Auto-induction/Medium 3: Turbo broth+ Augmedium from AthenaES™ Buffer 1: 40 mM HEPES;150 mM NaCl;10%glycerol;pH7/Buffer 2: 40 mM HEPES;400 mM NaCl;100 mM urea;pH7/Buffer 3: PBS pH8/Buffer 4: 40 mM Tris;150 mM NaCl;10%glycerol;pH8.5/Buffer 5: 40 mM Tris;400 mM NaCl;100 mM urea;pH8.5
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