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Table 1 :

From: Monitoring protein expression levels in E. coli using a high throughput approach

 

Expression strain

Culture medium

Lysis and purification buffer

Condition 1

Strain 2

Medium 2

Buffer 1

Condition 2

Strain 3

Medium 1

Buffer 4

Condition 3

Strain 3

Medium 2

Buffer 2

Condition 4

Strain 1

Medium 1

Buffer 1

Condition 5

Strain 2

Medium 3

Buffer 3

Condition 6

Strain 1

Medium 3

Buffer 4

Condition 7

Strain 2

Medium 2

Buffer 4

Condition 8

Strain 1

Medium 2

Buffer 3

Condition 9

Strain 3

Medium 2

Buffer 5

Condition 10

Strain 1

Medium 1

Buffer 5

Condition 11

Strain 2

Medium 3

Buffer 5

Condition 12

Strain 3

Medium 1

Buffer 3

Condition 13

Strain 3

Medium 3

Buffer 1

Condition 14

Strain 2

Medium 1

Buffer 2

Condition 15

Strain 1

Medium 3

Buffer 2

  1. Sparse matrix design (http://www.igs.cnrs-mrs.fr/samba/samba.html): Strain 1: BL21(DE3)/Strain 2: C43(DE3)(from OverExpress™)/Strain3: BL21(DE3) harboring pG-KJE8 vector from Takara Medium 1: LB/Medium 2: Auto-induction/Medium 3: Turbo broth+ Augmedium from AthenaES™ Buffer 1: 40 mM HEPES;150 mM NaCl;10%glycerol;pH7/Buffer 2: 40 mM HEPES;400 mM NaCl;100 mM urea;pH7/Buffer 3: PBS pH8/Buffer 4: 40 mM Tris;150 mM NaCl;10%glycerol;pH8.5/Buffer 5: 40 mM Tris;400 mM NaCl;100 mM urea;pH8.5