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Figure 2 | Microbial Cell Factories

Figure 2

From: Over-expression and single-step purification of human IFNα8 and human IFNα2b reveals the highest antiviral activity of human IFNα8

Figure 2

Expression of both HuIFNα subtypes in E. coli cells grown in minimum media. (A) 12.5% SDS-PAGE stained with Coomassie blue, and (B) Immunoblotting analysis using a mixture of rabbit polyclonal antibodies specific for both HUIFNα2 and HUIFNα8. Samples: (1) E. coli BL-21/ pALF8-4, (2) E. coli BL-21CP RIL/ pALF8-4, (3), E. coli BL-21CP RIL/ pAGUA-4, (4) E. coli BL-21/ pAGUA-4, (5) E. coli BL-21CP RIL. The molecular weight markers (M) are indicated (M).

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