Figure 1From: Disruption of the GAS1 gene of Pichia pastoris confers a supersecretory phenotype for Rhizopus oryzae lipase, but not for human trypsinogenA. P. pastoris GS115 wt cells showing the normal budding phenotype. B. P. pastoris GS115 GAS1::kanMX4. The GAS1 deleted P. pastoris strain shows frequently two buds attached to the mother cell (Mickey Mouse-like appearance) as reported for GAS1 deficient S. cerevisiae. The typical appearance is indicated by red circles.Back to article page