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Table 1 Comparison of typical max. cell dry masses, achieved product concentrations, specific product formation rates and volumetric productivities of the selected model processes.

From: Potentials and limitations of prokaryotic and eukaryotic expression systems for recombinant protein production – a comparative view

  CHO cells P. pastoris P. pastoris E. coli E. coli
Destination of product secreted secreted cytoplasm secreted cytoplasm
[g·L-1] 0.5 – 5 80 – 150 80 – 150 20 35
Typical spec. growth rate [h-1] 0.02 0.02 0.02 0.1 0.1
Typical specific product formation rate [mg·g-1·h-1] 1.5 – 4.5 0.05 – 0.5 4 0.25 – 2.5 20
Product concentration (per culture volume) [g·L-1] 0.1 – 0.5 0.25 – 2.5 20 0.2 – 2 7
Volumetric productivity [mg·L-1·h-1] 1 – 2 (- 25 for perfusion) 1 – 6 (- 25 for continuous processes) 160 4 – 40 200