Table 1 Comparison of typical max. cell dry masses, achieved product concentrations, specific product formation rates and volumetric productivities of the selected model processes.
CHO cells | P. pastoris | P. pastoris | E. coli | E. coli | |
|---|---|---|---|---|---|
Destination of product | secreted | secreted | cytoplasm | secreted | cytoplasm |
[g·L-1] | 0.5 – 5 | 80 – 150 | 80 – 150 | 20 | 35 |
Typical spec. growth rate [h-1] | 0.02 | 0.02 | 0.02 | 0.1 | 0.1 |
Typical specific product formation rate [mg·g-1·h-1] | 1.5 – 4.5 | 0.05 – 0.5 | 4 | 0.25 – 2.5 | 20 |
Product concentration (per culture volume) [g·L-1] | 0.1 – 0.5 | 0.25 – 2.5 | 20 | 0.2 – 2 | 7 |
Volumetric productivity [mg·L-1·h-1] | 1 – 2 (- 25 for perfusion) | 1 – 6 (- 25 for continuous processes) | 160 | 4 – 40 | 200 |