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Table 1 Expression of 30 DUB proteins in the BEVS at 10-L scale and processed in a semi-automated mode.

From: Automation for higher throughput in protein expression: visions, facts and fictions

  Analysis Method AVERAGE RANGE Av. Yield [%]
Small Scale Expression [mg/L] ELISA 27 3 – 140  
Large Scale Expression [mg/l] ELISA 43 0 – 270  
Large Scale Expression [mg] ELISA 448 0 – 1400 100 %
Cross-flow yields [mg] ELISA 169 0 – 825 41 %
Pure protein yields [mg] HPLC 21 0 – 103 (16%)*
  1. * different analysis method