Automation for higher throughput in protein expression: visions, facts and fictions

Table 1 Expression of 30 DUB proteins in the BEVS at 10-L scale and processed in a semi-automated mode.

	Analysis Method	AVERAGE	RANGE	Av. Yield [%]
Small Scale Expression [mg/L]	ELISA	27	3 – 140
Large Scale Expression [mg/l]	ELISA	43	0 – 270
Large Scale Expression [mg]	ELISA	448	0 – 1400	100 %
Cross-flow yields [mg]	ELISA	169	0 – 825	41 %
Pure protein yields [mg]	HPLC	21	0 – 103	(16%)*

ISSN: 1475-2859