Skip to main content

Advertisement

Table 3 Glycation of antibody produced in the first, second, and third series of 2L bioreactor experiments. Each condition was evaluated in duplicate bioreactors. Data represents average ± standard deviation obtained from duplicate cultures.

From: Cell culture efforts to reduce glycation in recombinant humanized antibody

Experiment Clone Condition Glycation Final [Glucose]
1 2 Control 19 ± 2 % 2.9 ± 1.1 g/L
1 191 Control 17 ± 2 % 1.9 ± 0.4 g/L
1 274 Control 15 ± 1 % 1.4 ± 0.5 g/L
2 2 Control 16 ± 2% 1.8 ± 0.1 g/L
2 2 Partial Continuous Glucose Feed 10 ± 1% 0.8 ± 0.1 g/L
3 2 Control 19 ± 1% 2.2 ± 0.2 g/L
3 2 Full Continuous Glucose Feed 6 ± 0 % 0.5 ± 0.4 g/L