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Figure 1 | Microbial Cell Factories

Figure 1

From: Determination of plasmid content in eukaryotic and prokaryotic cells using Real-Time PCR

Figure 1

Variation of threshold cycle numbers using plasmid-free E. coli (left) and CHO cells (right) spiked with pDNA. In E. coli, 5 × 104 (□), 2.5 × 105 () and 3.5 × 106 () cells were spiked with 8.5 × 10-4 to 8.5 ng of pDNA. With CHO, 1.2 × 104 cells were spiked with pDNA masses ranging from 5 pg to 100 ng. A linear working range was obtained from 5 pg to 2.5 ng and was subsequently used for quantitation.

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