Figure 1
From: Cost-effective production of labeled recombinant proteins in E. coli using minimal medium
Expression of 27.5 kDa soluble recombinant protein (marked by arrow). Expression is similar for LB and minimal medium for shake flask cultures and the same induction time. Fermenter cultivation allows a higher expression level due to longer induction time. Soluble cell fractions were prepared by chemical/enzymatic lysis. Induction 40 μM IPTG at 20°. Lane 1. SeePlus 2+ MW ladder. Lane 2. Shake flask LB, time after induction 17 h. Lane 3. Shake flask, minimal medium, time after induction 17 h. Lane 4. Fermenter 2H, 15N 5-litre scale fermentation, minimal medium, time after induction 40 h.