Improvement of the energy metabolism of recombinant CHO cells by cell sorting for reduced mitochondrial membrane potential

Table 1 Numbers in brackets are percent of the corresponding value of the parental cell line

	C2F5	C2G12	C2G12 low	C2G12 high
Inoculation cell concentration [cells/ml]	0,2 × 10⁶	0,2 × 10⁶	0,2 × 10⁶	0,2 × 10⁶
Cell count after 4 days [cells/ml]	2,0 × 10⁶	0,8 × 10⁶	1,4 × 10⁶ (170)	0,5 × 10⁶ (61)
Specific growth rate [μ/day]	0,61	0,39	0,51 (131)	0,26 (67)
Specific Glucose uptake [pg/cell/day]	525	900	660 (73)	1170 (130)
Specific Glutamine uptake [pg/cell/day]	130	185	165 (88)	245 (132)
Specific Lactate production [pg/cell/day]	505	700	420 (60)	1075 (154)
Specific mAb production [pg/cell/day]	6,3	3,7	7,3 (195)	4,4 (118)
Rh123 fluorescence at 2 g/l glucose [rel.U]	300	455	360 (80)	510 (112)

ISSN: 1475-2859