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Figure 3 | Microbial Cell Factories

Figure 3

From: Cell engineering of Pseudoalteromonas haloplanktis TAC125: construction of a mutant strain with reduced exo-proteolytic activity

Figure 3

Gelatin zymography of Ph TAC125 wt and gsp- supernatants. Psychrophilic cells were grown in TYP medium at 4°C till late exponential phase. Culture supernatants were recovered by culture centrifugation, 10 times concentrated and loaded onto a 10% SDS-PAGE containing bovine gelatin. After the electrophoresis run, the gel was washed to remove the Na-SDS and incubated in the development solution overnight at 15°C. Finally the gel is stained with Comassie blue and destained. Molecular weight markers were marked in kDa.

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