Figure 2From: Increasing the ease and speed of eukaryotic protein expression: a new cell-free in vitro translation system based on Sf insect cell extractsAkt1 was expressed in triplicate reactions using the EasyXpress Protein Synthesis Insect Kit. 2.5 μl aliquots were separated by SDS-PAGE and protein detected by Immunoblotting using an antibody specific for akt1 phosphorylated at Ser 473. NTC: no template control; M: marker.Back to article page