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Table 1 Parameters for mean yield coefficient calculation at 10 and 300 L scale fermentation.

From: Set up and optimization of a fermentation protocol for the production of a human antibody fragment (Fab') express in E. coli. Pre-pilot and cGMP pilot scale studies

Fermentation

Time

OD600

DCW

Substrate

Fab

Fab

DCW

Substrate

YXS

YPX

YPS

scale (l)

(h)

 

g.l-1

g.l-1

g.l-1

g.l-1.h

g.l-1.h

g.l-1.h

g.g-1

g.g-1

g.g-1

   

(X)

(S)

(P)

(rP)

(rX)

(-rS)

   

10

36

137

36.4

186

0.0642

0.00178

1.01

5.16

0.196

0.0018

0.00035

300

           

3

8

10

2.9

15

  

0.36

    

30

6

18

5.8

30

  

0.96

    

300

37

136

36.2

190

0.0685

0.00185

0.98

5.13

0.190

0.0019

0.00036