Table 2 hPRL periplasmic secretion level in E. coli W3110, at different temperatures utilizing a vector containing (phPRL-DsbA-cIts -λPL) and one not containing (phPRL-DsbA-λPL) the repressor gene.
temperature | phPRL-DsbA-λPL+ pRK-248 cIts (μg/mL/A600 ± SD) | phPRL-DsbA-λPL(μg/mL/A600± SD) | Statistical significance a |
|---|---|---|---|
30°C | 0.001 (n = 1) | 0.14 ± 0.02 (n = 6) | - |
35°C | - | 0.73 ± 0.07 (n = 5) | P < 0.001 |
37°C | 0.03 (n = 1) | 0.92 ± 0.10 (n = 6) | P < 0.01 |
39°C | - | 0.60 ± 0.12 (n = 8) | P < 0.001 |
42°C | 0.02 (n = 1) | 0.19 ± 0.05 (n = 4) | P < 0.001 |