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Figure 4 | Microbial Cell Factories

Figure 4

From: A novel genetic system for recombinant protein secretion in the Antarctic Pseudoalteromonas haloplanktis TAC125

Figure 4

Production, cellular localization and enzymatic activities of AmyΔCt-BlaM chimera in P. haloplanktis TAC125 recombinant cells. a Western Blotting analysis of extra-cellular media (lanes 2, 4, 6) and corresponding periplasmic extracts (lanes 1, 3, 5) of P. haloplanktis TAC125(pFFamyΔ Ct-blaM) recombinant cells. Samples were collected at the indicated times. The immunodetection was performed by using anti-α-amylase polyclonal antiserum. ref, AmyΔCt protein. b (▲) P. haloplanktis TAC125(pFFamyΔ Ct-blaM) liquid growth profile, () α-amylase enzymatic activity, and (□) β-lactamase enzymatic activity recovered in the extra-cellular medium.

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