Figure 3
Production and cellular localization of AmyΔCt-IGPS chimera in P. haloplanktis TAC125. a Western Blotting analysis of extra-cellular media (lanes 1 to 3) and corresponding periplasmic extracts (lanes 4 to 6) of P. haloplanktis TAC125(pFFamyΔ Ct-trpC) recombinant cells. Samples were collected during the growth at early, middle, and late exponential phases. The immunodetection was performed by using anti-α-amylase polyclonal antiserum. ref, AmyΔCt protein. b Western Blotting analysis of extra-cellular medium (lanes 1 to 3) of P. haloplanktis TAC125(pFFamyΔ Ct-trpC) performed using anti-Ss IGPS polyclonal antiserum. trIGPS, truncated form of Ss IGPS. ref, Ss IGPS protein.