Figure 2

Production and cellular localization of AmyΔCt-DsbA chimera in P. haloplanktis TAC125. a Western Blotting analysis of extra-cellular media (lanes 1 to 3) and corresponding periplasmic extracts (lanes 4 to 6) of P. haloplanktis TAC125(pFFamyΔ Ct-dsbA) recombinant cells. Samples were collected during the growth at early, middle, and late exponential phases. The immunodetection was performed by using anti-α-amylase polyclonal antiserum. ref, AmyΔCt protein. b Western Blotting analysis of extra-cellular media (lanes 1 to 3) of P. haloplanktis TAC125(pFFamyΔ Ct-dsbA) recombinant cells performed using anti-Ph DsbA polyclonal antiserum. ref, Periplasmic extract of non-recombinant P. haloplanktis TAC125 cells, which contains endogenous DsbA.