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Table 5 Primers for production for in vitro transcription templates

From: Antisense RNA based down-regulation of RNaseE in E.coli

Primer

Sequence 5'-3'1

Annealing temperature

Template

rne amp fw

ctaatacgactcactatagggaga CAACGCAACTCAGCAGGAAG

47°C

Chromosomal DNA E.coli

rne amp rv

CGATCTGGTAGTGGCTGAAC

47°C

 

anti6 amp fw

ctaatacgactcactatagggaga AGCTCCAGATCTCGATATCC

50°C

pANTI6

anti6 amp rv

CGCTCTAGAAGTCGTCAATG

50°C

 
  1. 1 T7 – promoter sequences are underlined, sequence homologies are shown in capital letters.