Figure 3
From: Antisense RNA based down-regulation of RNaseE in E.coli
Quantitative detection of antisense 6 mRNA by FSHA. A)Growth curve of the culture used for sampling. B)Standard curve with antisense6 RNA in vitro transcripts applied for quantification of calculation of the cellular amount of antisense6 mRNA in cell extracts. The equation for the best fit of the curve is displayed and the R2 value. Error bars show the standard deviation of three parallel samples. The critical level for the detection signal is indicated by a horizontal line. C)Detected antisense6 mRNA molecules per cell. Error bars indicate the standard deviation of two parallel mRNA detections in different amounts of cell extracts (2.5 and 5 × 106 cells). Induction was performed at t = 0 min with aTc.