Figure 5From: High yield recombinant penicillin G amidase production and export into the growth medium using Bacillus megateriumCultivation and PGA production in microtiter plates. YYBm1 carrying pRBBm49 (encoding SP lipA -PGA) was cultivated in LB medium using microtiter plates and shaking flasks. OD578nm from microtiter plate cultivation was measured with a spectrophotometer and Multiskan Ascent photometer. PGA activity measurements were performed as described in material and methods.Back to article page