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Figure 1 | Microbial Cell Factories

Figure 1

From: High yield recombinant penicillin G amidase production and export into the growth medium using Bacillus megaterium

Figure 1

Influence of calcium ions on PGA production and export in B. megaterium. MS941 carrying pRBBm23 (encoding SP pga -PGA) was cultivated in LB medium with indicated concentrations of CaCl2. Proteins from 1.5 mL cell-free growth medium were precipitated by ammonium sulfate, analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue G250. Lane M shows Precision Plus Protein Standard (Bio-Rad, Muenchen, Germany).

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Microbial Cell Factories

ISSN: 1475-2859

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