Microbial Cell Factories

Table 6 Levels of amino acids and nucleotides in E. coli strains BL21, BL21/pOri1 and BL21/pOri2 cultured in the chemostat system at different growth rates.

From: Effects of the presence of ColE1 plasmid DNA in Escherichia coli on the host cell metabolism

	Biomass (mmol g^-1)
Component	Various growth rates^a			0.2 h^-1
	BL21	BL21/pOri1	BL21/pOri2	All strains
Ala	0.940	0.976	0.995	0.996
Arg	0.465	0.511	0.529	0.531
Asp	0.620	0.465	0.438	0.43
Asn	0.465	0.474	0.474	0.474
Cys	0.146	0.146	0.146	0.146
Glu	0.520	0.529	0.538	0.539
Gln	0.566	0.438	0.374	0.373
Gly	0.849	0.411	0.319	0.318
His	0.146	0.164	0.173	0.175
Ile	0.438	0.447	0.474	0.476
Leu	0.547	0.575	0.630	0.63
Lys	0.538	0.566	0.566	0.567
Met	0.237	0.328	0.356	0.357
Phe	0.310	0.401	0.411	0.412
Pro	0.347	0.438	0.438	0.438
Ser	0.438	0.529	0.529	0.539
Thr	0.493	0.575	0.575	0.576
Trp	0.109	0.128	0.164	0.165
Tyr	0.255	0.347	0.319	0.32
Val	0.693	0.675	0.675	0.661
dATP	0.782	0.782	0.782	0.782
dTTP	0.782	0.782	0.782	0.782
dCTP	0.807	0.807	0.807	0.807
dGTP	0.807	0.807	0.807	0.807
ATP	0.807	0.807	0.807	0.807
UTP	0.665	0.665	0.665	0.665
CTP	0.616	0.616	0.616	0.616
GTP	0.992	0.992	0.992	0.992

^a The following growth rates were determined when bacteria were cultured in the batch cultivation: BL21, 0.46 h^-1; BL21/pOri1, 0.39 h^-1; BL21/pOri2, 0.29 h^-1.

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ISSN: 1475-2859

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